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Functional characterization of ω9 fatty acid desaturase in oleaginous fungus Mortierella alpina by homologous expression
Kai Du 1 #,Haiqin Chen 1 *,Guangfei Hao 2,Xiaoyun Huang 1,Danhui Hao 1,Zhennan Gu 1,Hao Zhang 1,Wei Chen 2,YongQ Chen 1
1.State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, P.R. China
2. Synergistic Innovation Center for Food Safety and Nutrition, Wuxi 214122, P.R. China
*Correspondence author
#Submitted by
Subject:
Funding: the National Natural Science Foundation of China (No.No. 21276108, 31271812), the Fundamental Research Funds for the Central Universities)
Opened online:25 March 2014
Accepted by: none
Citation: Kai Du,Haiqin Chen,Guangfei Hao.Functional characterization of ω9 fatty acid desaturase in oleaginous fungus Mortierella alpina by homologous expression[OL]. [25 March 2014] http://en.paper.edu.cn/en_releasepaper/content/4590290
 
 
In oleaginous fungus Mortierella alpina, Δ9 fatty acid desaturases are the first key enzyme during unsaturated fatty acid biosynthesis. There are three Δ9 fatty acid desaturase genes in Mortierella alpina. In contrast to Δ9-I and Δ9-II, ω9 (Δ9-III) displayed negligible transcriptionally activity under culture conditions. Thus, the intrinsic function and its substrate specificity remain to be determined. In this work, the ω9 fatty acid desaturase from M. alpina ATCC 32222 was cloned and homologously over-expressed. Lipid analysis revealed that ω9 fatty acid desaturase had moderate activities using palmitic acid (C16:0) and stearic acid (C18:0) as substrates. Palmitoleic acid (C16:1) and oleic acid (C18:1) contents increased 3.25- and 1.95-fold in ω9 over-expressing clones compared to the wild-type strain. Low temperature (12 C) significantly improved the conversion rate of C16:0 to C16:1 (9.24-fold increase) by ω9 desaturation. Moreover, the ω9 fatty acid desaturase may have specific activities with very long-chain saturated fatty acid substrates since a small amount of C24:1 was detected in ω9 gene over-expressing strains but none in the wild-type strain.
Keywords:Mortierella alpina; Over-expression; ω9 fatty acid desaturase; substrate specificity
 
 
 

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