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Feeding glycerol-enriched yeast culture improves performance, energy status, and heat shock protein gene expression of lactating Holstein cows under heat stress
Liu Jin 1,Ye Gengping 1,Zhou Yuanli 1,Liu Yunhuan 1,Zhao Lina 1,Liu Yongjie 1,Chen Xingxiang 1,Huang Da 1 #,Liao Shengfa 2,Huang Kehe 1 * #
1.Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing 210095
2.Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, 39762
*Correspondence author
#Submitted by
Subject:
Funding: the Science and Technology Support Plan Project of Jiangsu Province (No.Grant No. BE2011364), Special Fundation of Public Sector Agricultural Research Project (No.from the Chinese Ministry of Agriculture, Grant No. 201003060), the research foundations of Mississippi Agricultural and Forestry Experiment Station (No.Grant Nos. 027000901200 and 027100351050)), the Doctoral Program of Higher Education of China (No.Grant Nos. 20110097110014 and 20120097130002)
Opened online: 9 May 2014
Accepted by: none
Citation: Liu Jin,Ye Gengping,Zhou Yuanli.Feeding glycerol-enriched yeast culture improves performance, energy status, and heat shock protein gene expression of lactating Holstein cows under heat stress[OL]. [ 9 May 2014] http://en.paper.edu.cn/en_releasepaper/content/4595742
 
 
In this study,the effects of supplemental common yeast culture (CY) and glycerol-enriched yeast culture (GY) on performance, plasma metabolites, antioxidant status, and heat shock protein 70 (HSP70) mRNA expression were evaluated in lactating Holstein cows under heat stress. During summer months, 30 healthy multiparous lactating cows (parity 3.25 ± 0.48; 60 ± 13 d in milk [DIM]; 648 ± 57 kg BW; an average milk yield of 33.8 ± 1.6 kg/d) were blocked by parity, previous milk yield, and DIM and randomly allocated to 3 dietary treatments: no supplemental yeast culture (Control), 1 L/d of CY (33.1 g yeast) per cow, and 2 L/d of GY (153.2 g glycerol and 31.6 g yeast) per cow. During the 60-d experiment, values of air temperature and relativehumidity inside the barn were recorded hourly every 3 d to calculate temperature-humidity index (THI). Weekly rectal temperatures (RT) and respiration rates and daily DMI and milk yield were recorded for all cows. Milk and blood samples were taken twice monthly, and BW and BCS were obtained on d 0 and 60. In this experiment, THI values indicated cows experienced a moderate heat stress. Cows supplemented with CY and GY had greater yields of milk, energy-corrected milk and milk fat, and milk fat percent but lower HSP70 mRNA expression in peripheral blood lymphocytes than Control cows (P < 0.05). Supplementing CY and GY tended (P < 0.15) to decrease RT at 1400 h, increase milk protein yield and erythrocyte glutathione, and reduce plasma urea nitrogen compared with Control. Lower plasma NEFA concentration and HSP70 mRNA expression in peripheral blood lymphocytes (P < 0.05) and tendencies towards greater plasma glucose concentration (P = 0.11) but less BW loss (P = 0.14) were observed in GY relative to CY cows. In conclusion, either CY or GY supplementation partially mitigated the negative effects of heat stress on performance and HSP70 mRNA expression of lactating cows, and GY supplementation provided additional improvements in energy status and HSP70 gene expression of lactating cows.
Keywords:Cow; glycerol; heat shock protein; heat stress; yeast culture
 
 
 

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