Home > Papers

 
 
Aerobic biodegradation of trichloroethylene by a bacterial community that uses hydrogen peroxide as the sole oxygen source
ZHANG Shiyang 1,WANG Xiaoli 2,ZHU Ruili 1,Li Hui 1 #,WANG Ping 2,YANG Jie 3,LIN Kuangfei 1,GU Jidong 4,LIU Yongdi 1 *
1.School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237
2.School of Biological Engineering, East China University of Science and Technology, Shanghai 200237
3.Research Institute of Wastes and Soil Remediation, Shanghai Academy of Environmental Sciences, Shanghai 200233
4.School of Biological Sciences, Swire Institute of Marine Science, The University of Hong Kong, Pokfulam Road, Hong Kong SAR
*Correspondence author
#Submitted by
Subject:
Funding: Specialized Research Fund for the Doctoral Program of Higher Education(No.20110074130002), Fundamental Research Funds for the Central Universities (No.222201313008), Shanghai Rising-Star Program (No.12QA1400800), Program for New Century Excellent Talents in University (No.NCET-13-0797), National Natural Science Foundation of China (No.No. 51378208, 41273109 and 41003031), Innovation Program of Shanghai Municipal Education Commission (No.14ZZ059)
Opened online:11 June 2014
Accepted by: none
Citation: ZHANG Shiyang,WANG Xiaoli,ZHU Ruili.Aerobic biodegradation of trichloroethylene by a bacterial community that uses hydrogen peroxide as the sole oxygen source[OL]. [11 June 2014] http://en.paper.edu.cn/en_releasepaper/content/4599609
 
 
In this study, a bacterial community was enriched to aerobically degrade trichloroethylene (TCE) by using hydrogen peroxide (H2O2) as the sole oxygen source. The enriched bacterial community could degrade 120 mg/L TCE within 12 d in the presence of 8 mM H2O2, which was found to be the optimum concentration, and the efficiency of TCE degradation reached up to 80.6%. The bacterial community adapted to 2-8 mM H2O2 by increasing the activities of key enzymes, including catalase, superoxide dismutase, and peroxidase. 16S rRNA gene cloning and sequencing showed that the dominant species in the community belonged to Bordetella, Stenotrophomonas, Sinorhizobium, Variovorax, and Sphingobium. Polymerase chain reaction analysis revealed that phenol hydroxylase (Lph) gene was involved in TCE degradation in the presence of H2O2. Phenol induced the bacterial Lph gene, which catalyzed epoxidation of TCE. Our study findings might contribute to the in situ bioremediation of TCE-contaminated groundwater.
Keywords:Environmental ecology; Chlorinated hydrocarbon; Bioremediation; Hydrogen peroxide; Oxygen supply; Bacterial community
 
 
 

For this paper

  • PDF (0B)
  • ● Revision 0   
  • ● Print this paper
  • ● Recommend this paper to a friend
  • ● Add to my favorite list

    Saved Papers

    Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded 293
Bookmarked 0
Recommend 5
Comments Array
Submit your papers