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Pear 14-3-3b Gene is Regulated during Fruit Development and Involved in Response to Salicylic Acid
SHI Haiyan * #,ZHANG Yuxing
College of Horticulture, Agricultural University of Hebei, Baoding 071001
*Correspondence author
#Submitted by
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Funding: the Research Fund for the Doctoral Program of Higher Education of China （No.Grant No. 20121302120004), the Hebei Province Science Foundation of China）
Opened online: 5 November 2014
Accepted by: none
Citation: SHI Haiyan,ZHANG Yuxing.Pear 14-3-3b Gene is Regulated during Fruit Development and Involved in Response to Salicylic Acid[OL]. [ 5 November 2014] http://en.paper.edu.cn/en_releasepaper/content/4616104
 
 
Plant 14-3-3 proteins (14-3-3s) are known to function in protein-protein interactions that mediate signal transduction pathways regulating many biological processes. The cDNA encoding putative 14-3-3 protein was isolated from pear (Pyrus pyrifolia) and designated Pp14-3-3b. Using PCR amplification technique, the genomic clone corresponding to Pp14-3-3b was isolated and shown to contain six introns. Phylogenetic analysis clearly demonstrated that Pp14-3-3b was classified into non-ε class of 14-3-3 super-families. Quantitative RT-PCR analysis indicated that the expression of Pp14-3-3b gene was developmentally regulated in fruit. Further study demonstrated that Pp14-3-3b expression was inhibited by salicylic acid (SA) in pear fruit. These data suggested that Pp14-3-3b might be involved in response to SA signaling during fruit ripening and senescence of pear.
Keywords:Pear (Pyrus pyrifolia); 14-3-3; Salicylic acid; Gene expression; Fruit development3
 
 
 

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