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Characterization of Calcium and Zinc Spatial Distributions at the Fibrocartilage of Bone-tendon Junction by SR-μXRF combined with BEI
Lu Hongbin 1 #,Chen Can 1,Wang Zhanwen 1,Xu Daqi 1,Zhou Jingyong 1,Zheng Cheng 1,Hu Jianzhong 2 *
1.Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, People’s Republic of China.
2.Research Center of Sports Medicine & Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha 410008, People’s Republic of China.
*Correspondence author
#Submitted by
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Funding: The Specialized Research Fund for the Doctoral Program of Higher Education （No.NO. 20110162110068）
Opened online:26 November 2014
Accepted by: none
Citation: Lu Hongbin,Chen Can,Wang Zhanwen.Characterization of Calcium and Zinc Spatial Distributions at the Fibrocartilage of Bone-tendon Junction by SR-μXRF combined with BEI[OL]. [26 November 2014] http://en.paper.edu.cn/en_releasepaper/content/4618019
 
 
Tendon attaches to bone through a functionally graded fibrocartilage zone, including uncalcified fibrocartilage, tidemark and calcified fibrocartilage; those zones play a pivotal role in relaxing load transfer between otherwise structurally and functionally distinct tissue types. Calcium and zinc are believed to play important roles in the normal growth, mineralization and repair of the fibrocartilage of bone-tendon junction (BTJ), yet the calcium and zinc spatial distributions at the fibrocartilage of the BTJ and their distribution-function relationship are not totally understood. Thus, synchrotron radiation-based micro X-ray fluorescence analysis (SR-μXRF) in combination with backscattered electron imaging (BEI) was employed to characterize the distributions of calcium and zinc at the fibrocartilage of patella-patellar tendon complex (PPTC). For the first time, the unique distributions of calcium and zinc at the fibrocartilage of the PPTC were clearly mapped by this method. We found that the distributions of calcium and zinc at the fibrocartilage of the PPTC were inhomogeneous. A significant accumulation of zinc was exhibited in the tidemark (TM) fibrocartilage zone. The Zn content in the TM was 3.17 times higher than that of the patellar tendon. The calcium content began to increase near the TM and increased exponentially across the calcified fibrocartilage region toward the patella. The highest calcium content (43.14 times of that of patellar tendon) was in the transitional zone of calcified fibrocartilage and the patella, approximately 69 μm from the location with the highest zinc content. This study indicated that the calcium and zinc spatial distributions were completely different and that SR-μXRF with BEI could accurately determine the characteristics of the calcium and zinc distributions at the fibrocartilage zone of the PPTC. The characterizations of the calcium and zinc spatial distributions elucidated in this study provide critical insights into the distribution-function relationships for calcium and zinc at the fibrocartilage zone and the regeneration/repair of this complex tissue system.
Keywords:Sports medicine; Bone-tendon junction; Calcium distribution; Zinc distribution; Synchrotron radiation-based micro X-ray fluorescence analysis; Backscattered electron imaging.
 
 
 

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