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Isolation and Characterization of Soybean Straw Lignin by Using LiCl/DMSO Solvent System
LIU Zhulan #,CAO Yunfeng *,WANG Zhiguo,SHI Liuxin
Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037
*Correspondence author
#Submitted by
Subject:
Funding: The Specialized Research Fund for the Doctoral Program of Higher Education of China （No.201232041100012）, Jiangsu Provincial Graduate Student Innovation Research Project （No.CXLX11_0529）, The National Natural Science Foundation of China （No.31200444）, The Doctorate Fellowship Foundation of Nanjing Forestry University（No.2012）, Priority Academic Program Development of Jiangsu Higher Education Institutions （No.PAPD）
Opened online:23 December 2014
Accepted by: none
Citation: LIU Zhulan,CAO Yunfeng,WANG Zhiguo.Isolation and Characterization of Soybean Straw Lignin by Using LiCl/DMSO Solvent System[OL]. [23 December 2014] http://en.paper.edu.cn/en_releasepaper/content/4623597
 
 
To improve the yield of cellulolytic enzyme lignin (CEL) from soybean straw and to minimize the structural changes during isolation, the dissolution-regeneration of ball-milled soybean stem in a lithium chloride (LiCl)/dimethyl sulfoxide (DMSO) solvent was applied prior to enzymatic hydrolysis. The regenerated stem was enzymatically hydrolyzed for 72 h to remove the carbohydrates and then extracted with 1,4-dioxane to obtain the cellulolytic enzyme lignin from dissolved-regenerated stem (DR-CEL). As expected, the yield of DR-CEL was much higher than that of CEL. With 2 h of ball-milling, the yield of DR-CEL was 40.1% (based on the total lignin). As the ball-milling time increased to 4 h, the yield of DR-CEL improved up to 82.4%, which was much higher than that of CEL with a yield of 26.0%. All the isolated lignin preparations were characterized using UV, FT-IR, 1H-NMR and 1H-13C HSQC NMR. The results showed that the main structural characteristics of these two kinds of lignin (CEL & DR-CEL) were extremely similar, except for a slightly higher amount of carbohydrates in DR-CEL. As a result, the combination of total dissolution-regeneration and enzyme hydrolysis of ball-milled lignocellulose has a great potential in isolating virgin lignin with relatively high yield and minimal structural changes.
Keywords:Pulp and paper making engineering; Soybean straw; LiCl/DMSO; Lignin isolation; Dissolution; Regeneration
 
 
 

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