Home > Papers

 
 
Effects of LIV1 expression on the proliferation of epithelial ovarian cancer cells to cisplatin
MA Xiaoli #,DUAN Hua *
Gynecological Minimal Invasive Department, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100006, China
*Correspondence author
#Submitted by
Subject:
Funding: none
Opened online:23 December 2014
Accepted by: none
Citation: MA Xiaoli,DUAN Hua.Effects of LIV1 expression on the proliferation of epithelial ovarian cancer cells to cisplatin[OL]. [23 December 2014] http://en.paper.edu.cn/en_releasepaper/content/4624058
 
 
Drug resistance to cisplatin is the primary cause of death in patients with epithelial ovarian cancer. The aim of this study was to investigate the effect of LIV1 expression on sensitivity of epithelial ovarian cancer cells SKOV3 to cisplatin. We tested the induction of LIV1 in epithelial ovarian cancer cells SKOV3 after cisplatin treatment by real-time PCR and western blot. We investigated the effect of LIV1 expression on sensitivity of epithelial ovarian cancer cells SKOV3 to cisplatin by MTT assay. Finally, we analyzed the mechanism of LIV1 in ovarian cancer cells SKOV3 by western blot. The level of LIV1 mRNA and protein was significantly upregulated after cisplatin treatment. The proliferation rate of ovarian cancer cells SKOV3 transfected with AS-LIV1(PCEP4 carrying antisense LIV1 cDNA) were obviously higher than control detected by MTT assays. Using Western Blot, we demostrated that the inhibition of cisplatin-induced apoptosis by knockdown of LIV1 might be associated with decreased endogenous levels of Bcl-2.The data presented in this report may contribute to understanding the molecular mechanisms of drug resistance to cisplatin, LIV1 may be a potential molecular target.
Keywords:Ovarian cancer; LIV1; Drug resistance; Cisplatin
 
 
 

For this paper

  • PDF (0B)
  • ● Revision 0   
  • ● Print this paper
  • ● Recommend this paper to a friend
  • ● Add to my favorite list

    Saved Papers

    Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded 67
Bookmarked 0
Recommend 5
Comments Array
Submit your papers