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Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China
Nonlinear optical single-molecular image technique is a new technique which is not widely recognized in scientific community. It is a new innovative principle to get a profile image of tiny materials noninvasively. by a series of lenses which diameters from small to large, adjusting each lens to move back or forward carefully along a straight line, the two different direction rays from the same point of the out edge of objects can focus on a plate to form an image. This technique can magnify profile images of small samples. Its x-y resolution breakthrough the limit of Abbe's diffraction law. Nowadays , its resolution can reach 1-5 nanometer . In this paper, we gave more supporting evidences that Nonlinear optical single-molecular image technique is a practical tool. This paper showed the evidences of application in DNA Double helix nanostructure study. The photos of our experimental results demonstrated that the principle of Nonlinear optical single-molecular image technique is correct. This technique will bring human a lot of knowledge and information about molecules, especially about macromolecules in living cell.
Keywords:optical microscopy; single-molecularimage; DNA helix