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Immunoglobulin A Expression Correlated with Aberrant Proliferation of Esophageal Epithelium
Huang Bo 1 #,Wang Jihong 2,Hu Bin 3,Wu Xianying 1,Lin Wenting 1,Tian Dongping 1,Su Min 1 *,Guo Dan 1
1.Department of Pathology & Institute of Clinical Pathology, The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou 515031, Guangdong Province, China
2.Department of Pathology, Luohe Medical College, Luohe 462002, Henan province, China
3.Department of Dermatology, Wuhan No.1 Hospital, Wuhan 430022, China
*Correspondence author
#Submitted by
Subject:
Funding: National Natural Science Foundation of China (No.NSFC)-Guangdong Joint Fund), grants of National Natural Science Foundation of China (No.No. 81101548 and 31171226)), Specialized Research Fund for the Doctoral Program of Higher Education of China (No.New Teachers, No.20114402120006)
Opened online:23 November 2015
Accepted by: none
Citation: Huang Bo,Wang Jihong,Hu Bin.Immunoglobulin A Expression Correlated with Aberrant Proliferation of Esophageal Epithelium[OL]. [23 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4661655
 
 
Many research groups have recently shown that various cancer types can express immunoglobulin, but investigation of immunoglobulin A, the human most abundant isotype of immunoglobulin and the most prominent antibody at mucosa, expression in esophageal squamous cell carcinoma has been lacking. In this study, IgA protein expression was examined by immunohistochemistry in 197 esophageal tissues including normal, precancerous and squamous cancer, and found that IgA protein expressed in all normal and Esophageal Intraepithelial Neoplasia (EIN) and 96.30% of esophageal cancer (EC) (p > 0.05), but the strong expression (++) rate of IgA in cancer (79.01%) was significantly lower than that of normal (90.12%, p = 0.048) and EIN (95%, p = 0.012). Furthermore, the expression pattern changed from cell membrane in normal to cytoplasm in EIN and cancer. From normal, low EIN to high EIN, with increasing of proliferation, IgA expression had an increasing tendency. In EC, IgA expression positively correlated with Ki-67 immunochemically labeled proliferation (rs = 0.223,p = 0.046) but not other clinicopathological parameters. Ki67 labeling index of IgA strong expression (++) group was significantly higher than that of IgA weak expression (+) group (p = 0.011). To avoid the inference of secretory IgA protein from plasma cell, we validated IgA expression by detecting its protein and messenger RNA meanwhile in EC cell lines (EC109) used immunofluorescence and nested Real-time PCR, respectively. Our results indicated that the capability of EC in producing IgA and IgA expression correlated well with aberrant proliferation of EC.)
Keywords:immunoglobulin A; esophageal cancer; esophageal intraepithelial neoplasia; Ki67; immunostaining
 
 
 

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