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| In this paper, the esterifications of phytosterol with oleic acid catalyzed by Candida rugosa lipase in organic solvent were studied. The esterification conditions (temperature, substrate molar ratio, reaction time and catalyst amount) were optimized by response surface methodology (RSM). The phytoserol linoleic acid ester was also prepared via lipase-catalyzed. The lipid metabolic effect of equivalent intakes of phytoserol oleic/linoleic acid esters and that of different intakes of the same ester were compared. The results demonstrated that the highest phytoserol oleic acid ester esterification degree of 77.43% was obtained under the optimized conditions: temperature of 41℃, reaction time of 19h, substrate molar ratio (oleic acid: phytosterol) of 2.4:1 and catalyst amount of 7%. The chemical structure of phytoserol oleic acid ester was confirmed by FT-IR and GC-MS. The prepared phytosterol esters decreased significantly TC, LDL-C, LDL-C/HDL-C, ApoAⅠ, ApoAⅠ/ApoB in serum and liver weight, HI, hepatic TC, hepatic TG, while they also increased significantly HDL-C and ApoB in serum. No significant changes were found in the concentrations of TG. In contrast, linoleic acid ester could have a advantage effect on cholesterol-lowering and antioxidant capacity over oleic acid ester. |
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| Keywords:Lipid nutrient; Lipase-catalyzed; Phytosterol esters; Cholesterol-lowering; Antioxidant capacity |
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