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Sapoviruses (SaVs) belong to the family Caliciviridae and are related to gastroenteritis of humans and animals. These agents have been reported from several countries of the world. In this study, A total of 169 stool samples from pigs were collected from 4 pig farms located in Henan Province in central China from January to February 2015. Specimens were tested by reverse transcription polymerase chain reaction (RT-PCR) using the primers p289/p290 designed to detect the polymerase gene of caliciviruses, including both SaVs and noroviruses (NoV). Results showed that three of the four farms wre positive for SaVs and the positive rates were 12.8% 15.6%, and7.1%, respectively. Overall, fiften of 169 (8.9%) stool samples were positive for SaVs. Ten of distinct SaVs sequences were confrimed by sequencing. Phylogenetic analysis based on the partial sequences of the RNA-dependent RNA polymerase (RdRp) gene indicated that these SaVs were divided into five distinct genogroup, GIII, GVII, GVIII, and two potential new genogroup GIX and GX. Four strains belonged to GIII, three stains belonged to GVII, and one belonged to GVIII, GIX or GX, respectivily. No NoV infection were detected in the current study. To the best of our knowledge, this is the first report that GVIII, GIX and GX SaVs infection detected in Chian. Furthermore, the high genetic variability and prevalence of SaVs infection provides evidence that different genogroups of SaVs are circulating in Chinese pig herds. |
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Keywords:Sapoviruses; High Genetic diversity; genogroup |
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