The involvement of sirtuins during optic nerve injury of rats

MENG Pei; WEI Jiacong; LIANG Jiajian; WANG Jingying; ZHI Ye; CUI Qi; GENG Yiqun

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The involvement of sirtuins during optic nerve injury of rats

MENG Pei 1,WEI Jiacong 2,LIANG Jiajian 3,WANG Jingying 3,ZHI Ye 3,CUI Qi 3,GENG Yiqun 4 *

1.Shantou University Medical College, Shantou515000

2.University Medical Center Groninge, Groninge9700RB

3.Joint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou515000

4.Shantou University Medical College, Joint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou515000

*Correspondence author

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Funding: This work is supported National Natural Science Foundation of China （No.81200712)）

Opened online:11 January 2016

Accepted by: none

Citation: MENG Pei,WEI Jiacong,LIANG Jiajian.The involvement of sirtuins during optic nerve injury of rats[OL]. [11 January 2016] http://en.paper.edu.cn/en_releasepaper/content/4673675

Sirtuins protects cells from injury while 7 members may have different roles. In this study, we applied young rat optic nerve injury model to analyze the change of Sirt1-7 at different time point to better understand the role of Sirtuins during optic nerve injury. 12 week old adult male F344 rats were used (total n=36). Rats were divided into two groups randomly. One group underwent optic nerve cut and the other group underwent peripheral nerve-optic nerve graft (PN-ON graft) on the left eye. At the time point of 1 day, 3 day, 1 week, 2 week and 4 week, the rats were sacrificed. Retinas of both eyes were removed. Total RNA was extracted and first-strand cDNA was synthesized. Sirt1-7 and housekeeping β-actin quantitative real-time PCR was performed. The quantitative real time PCR profile showed that 7 members of Sirtuins of both groups had time period after surgery. Sirtuin family mRNA transcript levels increased following optic nerve injury with and without peripheral nerve grafting. Sirt1 showed a quite different transcription pattern from the rest of the members. Our data indicated that Sirt1 and Sirts 2-7, or just Sirt2, played opposing roles in optic nerve injury. Sirts 4 and 6 were the only Sirts higher in the PN-graft group, where neuronal survival should be higher, these results suggested that Sirts 4 and 6 played the predominant role for Sirts in neuroprotection or axon regeneration.

Keywords:Sirtuins; Optic nerve injury; Neuroprotection; Rat.

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