Ryk is involved in Wnt5a repulsive axon guidance of mouse rubrospinal tract

Duan Xin; Liu Yaobo

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Ryk is involved in Wnt5a repulsive axon guidance of mouse rubrospinal tract

Duan Xin #,Liu Yaobo *

Institute of Neuroscience, Soochow University, Suzhou 215123

*Correspondence author

#Submitted by

Subject:

Funding: National Natural Science Foundation （No.No.30871425）

Opened online: 5 May 2016

Accepted by: none

Citation: Duan Xin,Liu Yaobo.Ryk is involved in Wnt5a repulsive axon guidance of mouse rubrospinal tract[OL]. [ 5 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4685772

Objective: Red nucleus (RN) is a predominant neuronal population for forelimb skilled motor control and function, rubrospinal tract (RST) projects from RN and delivers signals to spinal cord targets. However little is known about the molecular mechanism underlying RST axon pathfinding in the developing spinal cord. Lots of studies have identified Wnt family proteins as axon guidance cues that control axon pathfinding along the anterior-posterior (A-P) axis. The aim of this study is to investigate whether Wnt signaling pathway plays a role in RST axon pathfinding in the spinal cord. Methods: Whole mount in situ hybridization was used to detect the expression pattern of Wnt5a during the RST axon descending. Immunohistochemistry was performed to detect whether Ryk was expressed in RN neurons. shRNA lentivirus was used to knock down Ryk expression level in RN neurons. Turning assay and Dunn chamber assay were performed to investigate the repulsive guidance of RST axons under Wnt5a gradient. Results: Wnt5a established a decreasing gradient along the A-P axis of the brainstem and spinal cord. Ryk, as the receptor of Wnt5a, was specific expressed in RN neurons during the RST axon descending. Wnt5a protein repelled RST axons and Ryk was involved in Wnt5a repulsion of RST axons. Conclusions: These findings indicate that Wnt5a/Ryk signaling pathway plays a role in the RST axon pathfinding along the spinal cord.

Keywords:Neurobiology; Red nucleus; Rubrospinal tract; Axon guidance; Wnt5a; Ryk

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