| |
| Yu-Ping-Feng is a well-known traditional Chinese multi herbal formula, and it is frequently being used to treat cold and flu and inflammation-associated diseases. We aim to evaluate the immunostimulatory activity of polysaccharide from Yu-Ping-Feng(YPF-PS) in vitro on chicken macrophage to make clear the bioactive molecular of Yu-Ping-Feng. Gel permeation chromatography (GPC) and gas chromatography?mass spectrometry (GC-MS) analysis, amino acid analysis and nuclear magnetic resonance (NRM) spectroscopy elucidated the structure of the YPF-PS. In order to evaluate the biological activities of YPF-PS, lipopolysaccharides (LPS)-induced cell proliferation and phagocytosis rate and the levels of cytokine: nitric oxide (NO), tumor necrosis factor alpha (TNF-α), interleukin-1 beta, interleukin-6 (IL-1β, IL-6), interferon beta (IFN-β) and costimulatory molecules CD80, CD86 in chicken macrophage were detected. The results indicated that molecular weight the weight of YPF-PS was distributed on 5 KD~500 KDa, which is a mixture of glucose (Glu), fructose (Fru), arabinose (Ara) and few rhamnose with the molar ratio of 61.29: 55.47: 99.67: 19.58, having anomeric carbon signals of β-L-arabino furanoside, α-L-rhamnoside, α-D-glucopyranoside and β-D-fructofuranose. Furthermore, cell immunology experiments show that YPF-PS at dosage of 15.62 μgmL -1 could increase the LPS-induced cell proliferation and phagocytosis rate significantly in chicken macrophage, moreover, the levels of cytokine: NO, TNF-α, IL-1β, IL-6 and IFN-βin cells elevated obviously, equally shocking, costimulatory molecules CD80, CD86 were detected having a tremendous enhancement. We believe that the YPF-PS has potent immunomodulatory properties and could be explored as a novel potential immunostimulant for the food and pharmaceutical purpose. |
|
| Keywords:Yu-Ping-Feng; Ppolysaccharide; Chicken macrophage activation; Immunological activity; Cytokine |
|
