| |
| This study evaluated the immunity-enhancing activity of polysaccharides from Platycodon Grandiflorum (PGPS) in vitro. Lymphocyte proliferation, cell cycle distribution, and percentages of CD4+ and CD8+ T cells were investigated. Different concentrations of PGPS (PGPStc, PGPS60c, PGPS80c and PGPStp) were added to peripheral blood T lymphocytes. Results showed that PGPStc markedly enhanced T lymphocyte proliferation individually or synergistically with phytohemagglutinin (PHA) at most concentrations and the lymphocyte proliferation rates were the highest. Then, the active sites of PGPStc and PGPS60c were subsequently selected. Lymphocyte cell cycle distribution and percentages of CD4+ and CD8+ T cells were determined by flow cytometry. At most time points, PGPStc could promote lymphocytes enter into S and G2/M phases, while increasing the percentages of CD4+ and CD8+ T cells. These results indicate that PGPStc enhances the immune functions, suggesting that PGPStc could be a potential immunopotentiator for further in vivo and clinical trial experiments. |
|
| Keywords: Platycodon Grandiflorum; Polysaccharides; Immunity-enhancing activity; Lymphocyte proliferation; Cell cycle |
|
