A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks

BAI Miao; CHEN Wenting; XIE Bingyan; YANG Guoshun

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A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks

BAI Miao 1 #,CHEN Wenting 1,XIE Bingyan 2,YANG Guoshun 1 *

1.College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, P.R. China

2.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China

*Correspondence author

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Funding: Specialized Research Fund for the Doctoral Program of Higher Education of China （No.20134320120013）, Natural Science Foundation of Hunan province （No.14JJ3095）

Opened online:18 May 2016

Accepted by: none

Citation: BAI Miao,CHEN Wenting,XIE Bingyan.A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks[OL]. [18 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4687477

Cucumber mosaic virus (CMV) can infect a wide range of host species. For the lacking of CMV-resistant varieties of tomato, RNA interference can be used as a fast and effective method for the generation of transgenic resistant varieties. In this current study, five intron-spliced hairpin RNA (ihpRNA) plant expression vectors aim at five genes of CMV has been constructed. Transgenic tomatoes were obtained by Agrobacterium mediated transformation with expression vectors. Highly resistant generations of transgenic plants were employed as rootstocks and grafted onto non-transgenic tomatoes that resulted in the successful transfer of resistance to the scions. Using a novel method of plant cuttings for rootstock propagation, we obtained large quantities of disease-resistant material. Further, this method produces scions that can remain undetectable for transgenic resistance marker genes that may provide novel approaches to evade collective concerns about genetically-modified organism (GMO) biosafety.

Keywords:Cucumber mosaic virus; graft; RNA silencing; Solanum lycopersicum; virus resistance

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