Neonatal Murine Keratinocytes Split Express CD80/CD86 upon Culture

LeiJianjun; ChengJingqiu; LiYouping; LiShengfu; ZhangLi; KeNengwen

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Neonatal Murine Keratinocytes Split Express CD80/CD86 upon Culture

LeiJianjun 1 #,ChengJingqiu 1,LiYouping 2 *,LiShengfu 1,ZhangLi 1,KeNengwen 1

1.Key Lab of Transplant Engineering and Immunology MOH,West China Hospital

2.Key Lab of Transplant Engineering and Immunology MOH-West China Hospital

*Correspondence author

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Funding: the National Natural Science Foundation,the Specialized Research Fund for the Doctoral Program of Higher Education（No.30371355,20020610086）

Opened online:28 December 2005

Accepted by: none

Citation: LeiJianjun,ChengJingqiu,LiYouping.Neonatal Murine Keratinocytes Split Express CD80/CD86 upon Culture[OL]. [28 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4704

It was previously thought that keratinocytes did not express the CD80 and CD86 which provide the most important costimulatory signals for the antigen-specific T-cell activation.The cultured keratinocytes allografts were initially accepted,but eventually,allgrafted donor cells were gradually replaced by recipient cells.The precise mechanisms are not very clear.In this study,neonatal murine keratinocytes were cultured for 7 days,the results of flowcytometry and confocal microscopy showed that CD80 could be detected on keratinocytes,whileCD86 could not be detected all the time.RT-PCR analysis confirmed this result.The expression level of the CD80 mRNA amplified significantly from day1 to day 7,as expression of the control B-actin,but CD86 was not detected.Mixed Lymphocyte Reaction(MLR) showed that keratinocytes cultured with 10% serum for 7d stimulated effectively allogeneic rather than syngeneic T cell proliferation.This study demonstrated for the first time that co-stimulatory molecule CD80 can be expressed onkeratinocytes invitro.These data provided an alternative explanation for the ultimate rejection of allogeneic keratinocytes in which keratinocytes act as antigen-presenting cells.

Keywords:Co-stimulation T cell activation Rejection Keratinocytes

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