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Selenomethionine alleviates the AFB1-induced damages in primary cultured chicken hepatocytes by improving SelW expression to inhibit CYP450 1A5 expression
Chen Xingxiang #,Che Chaoping,Gan Fang,Pan Cuiling,Huang Kehe *
College of Veterinary Medicine, Nanjing Agricultural University
*Correspondence author
#Submitted by
Subject:
Funding: Specialized Research Fund for the Doctoral Program of Higher Education (No.20130097120025), The National Natural Science Foundation of China (No.NFSC)), The Priority Academic Program Development of Jiangsu Higher Education Institutions (No.Jiangsu, China))
Opened online: 1 December 2016
Accepted by: none
Citation: Chen Xingxiang,Che Chaoping,Gan Fang.Selenomethionine alleviates the AFB1-induced damages in primary cultured chicken hepatocytes by improving SelW expression to inhibit CYP450 1A5 expression[OL]. [ 1 December 2016] http://en.paper.edu.cn/en_releasepaper/content/4711502
 
 
The aim of this study was to evaluate the effects of selenomethionine (SeMet) on the hepatotoxicity induced by Aflatoxin B1 (AFB1) in primary cultured chicken hepatocytes and explore its mechanism involved. The results revealed that the toxic effect of AFB1 on chicken hepatocytes is dose dependent and AFB1 at concentration of over 0.05 μg/mL caused significant increases in the levels of malondialdehyde and cytochrome P450 enzymes (CYP450 1A5) mRNA and reduction on the levels of intracellular glutathione (GSH) and total superoxide dismutase in hepatocytes when compared with the control (P < 0.05). Supplement with 2 μmol/L of SeMet exerted protective effect against the AFB1-induced damage in chicken hepatocytes by significantly increasing the mRNA level of Selenoprotein W (SelW) and decreasing the mRNA level of CYP450 1A5 (P < 0.05). Knockdown of SelW with SelW-specific siRNA significantly increased the mRNA and protein levels of CYP450 1A5 (P < 0.05) and further attenuated the protective effect of SeMet on the AFB1-induced damage. Thus, it can be concluded that selenomethionine alleviates the AFB1-induced damage in primary cultured chicken hepatocytes by improving SelW expression to inhibit CYP450 1A5 expression.
Keywords:selenomethionine, AFB1, cytochrome P450 enzymes, siRNA, primary chicken hepatocytes.
 
 
 

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