Monochromatic green light stimuli during incubation accelerates posthatch pectorals muscle growth by altering the expression of myogenic genes and wnt signaling pathway of embryos and neonatal broilers

ZHANG Lin; LI Jiaolong; WANG Xiaofei; ZHU Xudong; GAO Feng

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Monochromatic green light stimuli during incubation accelerates posthatch pectorals muscle growth by altering the expression of myogenic genes and wnt signaling pathway of embryos and neonatal broilers

ZHANG Lin 1 #,LI Jiaolong 1,WANG Xiaofei 2,ZHU Xudong 2,GAO Feng 1 *

1.College of Animal Science and Technology, Jangsu Provincial Key Laboratory of Animal Origin Food Production and Safety Guarantee, Nanjing Agricultrual University, Nanjing 210095

2.College of Science, Nanjing Agricultural University, Nanjing 210095

*Correspondence author

#Submitted by

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Funding: Research Fund for the Doctoral Program of Higher Education of China （No.No. 20130097120051）

Opened online:24 February 2017

Accepted by: none

Citation: ZHANG Lin,LI Jiaolong,WANG Xiaofei.Monochromatic green light stimuli during incubation accelerates posthatch pectorals muscle growth by altering the expression of myogenic genes and wnt signaling pathway of embryos and neonatal broilers[OL]. [24 February 2017] http://en.paper.edu.cn/en_releasepaper/content/4719405

Previous studies have indicated that monochromatic green light-emitting diodes (LED) light stimuli during embryogenesis accelerated post-hatch pectoral muscle growth of broilers. In this study, we further investigated the potential mechanism of this phenomenon by determine the gene expression of paired box genes, myogenic regulatory factors and the key factors of Wnt/β-catenin signaling pathway in the pectoral muscle of embryos and neonatal broilers incubated in dark condition or green LED light stimulation. Arbor Acres fertile broiler eggs (n = 200) were randomly assigned to 2 incubation groups and exposed to continuous green light, or a dark condition (control) from the first day to 19 d of incubation. The light sourced from LED lamps with the intensity of 30 lx at eggshell level. The results showed that green LED light stimuli during embryogenesis increased the total pectorals muscle weight on days H3 and H7, and relative pectorals muscle weight of newly hatched chicks on day H7 (P < 0.05) in comparison to the dark condition. The expression of Pax3 on days E15 (P < 0.05) and E17 (P < 0.01), Pax7 on days E19 and H1 (P < 0.05), MyoD on days E19 and H1(P < 0.05), MyoG on day H1 (P < 0.05), and Myf5 from days E17 to H1 (P < 0.05) was higher in green light group than those in dark condition. In addition, although the no significant difference on Wnt11 mRNA expression was found between treatments, the green LED light stimuli upregulated the mRNA expression of Wnt3a form days E15 to E19, and β-catenin on day E19 (P < 0.05). These results suggest that providing 30 lx green LED light stimuli from the first day to 19 d of incubation may accelerate pectoral muscle growth in embryos and neonatal broilers by enhancing myoblast proliferation and differentiation in the late embryonic stage of embryos and neonatal broilers, which is potentially mediated through the transcriptional upregulation of Pax3, Pax7, MyoD, MyoG and Myf5 and the key factors (Wnt3a and β-catenin) of Wnt/β-catenin signaling pathway..

Keywords:chick embryo, incubation, green LED light, muscle development

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