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Enhanced production of natural yellow pigments from Monascus by liquid culture: A study of the fermentation conditions and mycelial morphology
Lv Jun 1,Zhang Bobo 1 *,Xu Gangrong 1,Cheung ChiKeung Peter 2
1.School of Biotechnology, Jiangnan university, Wuxi, 214122
2.School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
*Correspondence author
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Funding: the Priority Academic Program Development of Jiangsu Higher Education Institutions, the 111 Project(No.111-2-06), National Key Research and Development Program of China (No.2016YFD0400802), the Undergraduate Innovation Training Program of Jiangnan University of China(No.2016151Y)
Opened online: 1 March 2017
Accepted by: none
Citation: Lv Jun,Zhang Bobo,Xu Gangrong.Enhanced production of natural yellow pigments from Monascus by liquid culture: A study of the fermentation conditions and mycelial morphology[OL]. [ 1 March 2017] http://en.paper.edu.cn/en_releasepaper/content/4719755
 
 
Natural yellow pigments produced by submerged fermentation of Monascus purpureus have potential economic value and application in the food industry. In the present study, the relationships among fermentation conditions (in terms of pH and shaking/agitation speed), mycelial morphology and the production of Monascus yellow pigments were investigated in both shake-flask and scale-up bioreactor experiments. In the shake-flask fermentation, the highest yield of the Monascus yellow pigments was obtained at pH 5.0 and a shaking speed of 180 rpm. Microscopic images revealed that these results were associated with the formation of freely dispersed small mycelial pellets with shorter, thicker and multi-branched hyphae. Further investigation indicated that the hyphal diameter was highly correlated with the biosynthesis of the Monascus yellow pigments. In a scaled-up fermentation experiment, the yield of the yellow pigments (401 U/mL) was obtained in a 200-L bioreactor, which is the highest yield to the best of our knowledge. The present findings can advance our knowledge on the conditions used for enhancing the production of Monascus yellow pigments in submerged fermentation and facilitate large-scale production of these natural pigments.
Keywords:Monascus purpureus; Natural yellow pigment; Mycelial morphology; Submerged fermentation; Bioreactor
 
 
 

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