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| Increasing evidence has demonstrated the association between long noncoding RNAs (lncRNAs) and multiple autoimmune diseases. In this study, we explored four lncRNAs (GAS5, lnc-DC, linc0597 and linc0949) expression levels and gene polymorphisms in systemic lupus erythematosus (SLE). A two stage design was applied. 85 SLE patients and 71 healthy controls were enrolled to investigate the lncRNAs expression levelsin stages one. Then, 860 SLE patients and 831 healthy controls were included to detect the single nucleotide polymorphisms (SNPs) in the differentially expressed lncRNAs in stage two. Expression levels of lncRNAs were detected by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Genotyping was performed using the TaqMan SNP genotyping assays by an EP1 platform. The expression levels of linc0597, lnc-DC and GAS5 were significantly lower in SLE patients than healthy controls (Z=-5.984, P<0.001; Z=-3.703, P<0.001; Z=-2.995, P=0.003 respectively). No significant differences in linc0949 expression level was found between SLE patients and healthy controls (Z=-0.254, P=0.799). The expression levels of linc0597 was significantly decreased in lupus nephritis (LN) compared with SLE patients without nephritis (Z=-2.411, P=0.016). Moreover, the expression level of linc0597 was correlated with systemic lupus erythematosus disease activity. Four SNPs (rs10515177 for lnc-DC; rs2070107, rs2632516, rs2877877 for linc0597) with SLE risk were analyzed. Significant association was observed between the distribution of genotype (CC vs. GG) at SNP rs2070107 and susceptibility to SLE (OR=2.414, 95% CI: 1.266-4.601, P=0.007), and an increased risk was also found in the recessive model (CC vs. CG+GG) (OR=2.415, 95% CI: 1.271-4.590, P=0.007). Furthermore, linc0597 expression level may be associated with the dominant model (CC+CG vs GG) of rs2070107 in the exploration of 34 patients. The association between linc0597 SNPs and SLE as well as its altered expression and correlation with disease activity implicates an important role of this gene in the pathogenesis of SLE. |
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| Keywords:Epidemiology; Long noncoding RNA; Systemic lupus erythematosus; Polymorphisms |
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