| |
| To investigate the effect of different methionine sources on lipid deposit and lipid metabolism related genes expression in the liver of broilers during different growth phase, two trails was conducted. A total of 1200 1-day-old male Ross 308 broiler chicks with similar body weight were randomly selected and allotted to 10 treatments with 6 replications of 20 birds in trail 1. Birds were fed with either a corn-soybean meal-pea protein concentrate-based diet (Met：0.28, 1-21d with no methionine and cysteine supplementation), or methionine supplemented diets at 0.10, 0.22 and 0.32% from L-methionine (LM), DL-methionine (DLM)or methionine hydroxyl analogue calcium Salt (MHA-Ca) from 1-21d based on the Met content (LM or DLM) or equal mass to MHA. In trail 2, a total of 2112 21-day-old chicks were randomly allotted to 12 treatments with 8 replications of 22 birds each. Birds were fed with methionine supplemented diets at 0.05, 0.10, 0.14 and 0.24% from LM, DLM or MHA-Ca from 22-42d. A treatment fed with a basal diet (Met: 0.28, 1-21d; 0.26%: 22-42d) from 1-42d was set as negative control. Met deficiency significantly increased liver EE in broilers during 1-21d (P<0.0001) and Met excess increased that during 22-42d (P<0.0001). Chickens fed LM supplemental diets had the highest liver EE and MHA-Ca the lowest regardless of growth phase (P<0.0001).Met level had a significant effect on adiponectin receptor2 (ADIPOR2), acyl-coenzyme A oxidase 1 (ACOX1), carnitine palmitoyltransferase (CPT1A), carbohydrate response element-binding protein (CHREBP), fatty acid synthase (FASN) and apolipoprotein (APOB) expression during 22-42d (P<0.05) while only affected ACOX1, CPT1Aand CHREBP expression during 1-21d (P<0.05). There was a difference on CPT1A(P=0.010) and CHREBP (P=0.007) expression among Met sources during 1-21d and the expression of CHREBP(P=0.009), FASN (P=0.014) and APOB (P=0.002) existed difference during 22-42d. In conclusion, Met deficiency and excess may increase liver lipid accumulation by suppressing lipid catabolism and transport related gene expression in broilers and MHA-Ca showed a good benefit for fatty liver. |
|
| Keywords:Animal nutrition and feed science; methionine source; methionine level; lipid metabolism; gene expression; broiler liver |
|
