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A naphthalimide based fluorescent probe for imaging microviscosity in living cells
Guo Bingpeng 1,Zhang Xiaoling 2 * #
1. Beijing Institute of Technology, chemistry and chemical engineering, Beijing 100081
2.Beijing Institute of Technology, chemistry and chemical engineering, Beijing 100081
*Correspondence author
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Funding: Research Fund for the Doctoral Program of Higher Education of China (RFDP)(No.No. 20121101110049)
Opened online:28 April 2017
Accepted by: none
Citation: Guo Bingpeng,Zhang Xiaoling.A naphthalimide based fluorescent probe for imaging microviscosity in living cells[OL]. [28 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4728806
 
 
Intracellular viscosity strongly influences transportation of mass and signal, interactions between the biomacromolecules and diffusion of reactive metabolites in live cells. Herein, we developed a naphthalimide-hemicyanine based fluorescent probe Cyt-NA for imaging the change of microviscosity in living cells. Cyt-NA exhibited about 260 folds fluorescence increasing at 610 nm along with the increasing viscosity from 1.0 cP to 1410 cP. These satisfying response properties make Cyt-NA possible to monitor viscosity changes under starvation and low temperature conditions, offering us more practical information about microenviromental viscosity in living cells.
Keywords:fluorescent probe; microviscosity; cell imaging
 
 
 

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