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OsANN4 confers stress induced by ABA signaling and regulates antioxidant accumulation
Can Guan,Qian Zhang 1,Yingjie Gao 1,Jianchao Ma 1,Ying Zhu 2,Zhengge Zhu 1 * #
1.College of Life Science, Hebei Normal University, Shijiazhuang 050024
2.Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences,
*Correspondence author
#Submitted by
Subject:
Funding: National Natural Science Foundation of China (No.31571638), Research Fund for the Doctoral Program of Higher Education(No.20131303110005)
Opened online: 4 May 2017
Accepted by: none
Citation: Can Guan,Qian Zhang,Yingjie Gao,Jianchao Ma.OsANN4 confers stress induced by ABA signaling and regulates antioxidant accumulation[OL]. [ 4 May 2017] http://en.paper.edu.cn/en_releasepaper/content/4730556
 
 
Annexins are Ca2+-dependent phospholipids-binding proteins constituting evolutionary conserved multigene family that widely distributed throughout animals and plants. OsANN4 is a member of rice annexin protein family. However, the functions and mechanisms of OsANN4 in stress responses and signal pathways remain unclear. GUS staining assay and quantitative RT-PCR indicated that the expression of OsANN4 is tissue-specific. In this study, OsANN4 expression was high at seedling stage. The plants which knocking down OsANN4 through RNA interference were more sensitive to ABA at the seeds germination stage comparing to wild type. It suggested that OsANN4 was involved in response to ABA signaling under abiotic stress. Furthermore, yeast two-hybrid assays demonstrated that OsANN4 interacted with OsCDPK24. This may provide potential regulation cross-talk in the abiotic stress response.
Keywords:Molecular biology; annexin; calcium binding activity; ABA signaling; Oryza Sativa
 
 
 

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