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Identification of ZmaM, a bifunctional ABC transporter, reveals the presence of other alternative transport and peptide cleavage pathway during zwittermicin A biosynthesis in Bacillus cereus
LUO Yi 1 #,ZHANG Yuyang 2,SUN Ming 2 *
1.College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
2.College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
*Correspondence author
#Submitted by
Subject:
Funding: Specialized Research Fund for the Doctoral Program of Higher Education of China (No.20130146120021)
Opened online: 5 May 2017
Accepted by: none
Citation: LUO Yi,ZHANG Yuyang,SUN Ming.Identification of ZmaM, a bifunctional ABC transporter, reveals the presence of other alternative transport and peptide cleavage pathway during zwittermicin A biosynthesis in Bacillus cereus[OL]. [ 5 May 2017] http://en.paper.edu.cn/en_releasepaper/content/4730843
 
 
ZmaM, a possible ABC transporter maturation and secretion (AMS) protein, whose coding gene locates in the biosynthesis gene cluster of zwittermicin A (ZmA), a novel antibiotic produced by certain Bacillus cereus group strains, may contribute to the fatty acyl-D-Asn-ZmA intermediate transport, and to the cleavage of the acyl-D-Asn of the intermediate, releasing mature ZmA. AMS protein is common in the lantibiotic peptide biosynthesis but rare in the antibiotic biosynthesis. The peptidase activity of ZmaM has been identified in vitro, but the real contribution of ZmaM to the ZmA biosynthesis still unknowns. In this study, we detailedly demonstrated the structure of ZmaM, and constructed an ABC transporter domain and a whole zmaM gene in-frame deletion mutants respectively. Unexpectedly, both mutants were found still can produce ZmA, but the ZmA yields became almost half of the wild-type strain UW85. The transporter activity of ZmaM was confirmed and the contribution of ZmaM to ZmA biosynthesis was indentified due to these findings. Our results also indicates that there is sure existing other alternative transport and peptide cleavage pathway during ZmA biosynthesis. Assuredly, several genes which predicted could be complementary to the function of ZmaM were found in stain UW85 and another ZmA producing stain B. thuringiensis YBT-1520. These results make a further step to clarify the ZmA biosynthesis.
Keywords:Bacillus cereus; zwittermicin A; biosynthesis; transporter
 
 
 

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