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| Introduction Spinal Cord Ischemia-reperfusion Injury (SCII) is a common complication of spinal surgery and thoracic and abdominal surgery, lead to higher morbidity and mortality. Although the pathogenesis of SCII is not fully understood, the acute cytotoxic edema at the site of injury is the key cause of the SCII, and the degree of edema determines the prognosis of SCII. Therefore, avoiding spinal nerve cell edema is the key to the treatment of SCII. Methods The rat SCII model was established to elucidate whether ginsenoside Rb1 intervention could alleviate the edema of SCII in rats and further determine the effect of ginsenoside Rb1 on the expression and activity of AQP4. To establish the model of oxygen-glucose deprivation/reoxygenation（OGD/R）to simulate ischemia-reperfusion injury at the cellular level. The expression of AQP4 in OGD/R culture system was confirmed by RNA interference technique or rescue experiment. To determine the effect of ginsenoside Rb1 on the astrocytes edema in OGD/R cell culture conditions. Results In animal model, ginsenoside Rb1 could alleviate the edema of spinal cord and improve the neurological function by increasing the expression of AQP4. Further, at the cellular level, it can be concluded that ginsenoside Rb1 can reduce the astrocyte edema by increasing the expression of AQP4 and enhancing its function. Conclusion These findings demonstrate ginsenoside Rb1 can relieve spinal cord edema and improve neurological function by increasing AQP4 expression. |
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| Keywords:Surgery; Spinal cord ischemia-reperfusion injury; Edema; ginsenoside Rb1; AQP4 |
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