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Reductive soil disinfestation (RSD), alternatively named as biological soil disinfestation or anaerobic soil disinfestation, is proven to be an environmentally friendly method to control soil-borne disease. Microorganisms are regarded as the key contributors for RSD, however, the relative investigations about these microbe which was essential for the in deep understanding of RSD are deficient. In this study, RSD was performed in four soils, and the bacterial community during the RSD process was detected using pyrosequencing based on bacterial 16S rDNA. The results showed that RSD significantly changed soil bacterial community structure and reduced the bacterial richness and diversity. The relative abundance of Firmicute profoundly increased as an alternative of the decreases in the relative abundances of Actinobacteria and Acidobacteria. Ruminococcaceae, Lachnospiraceae and Clostridiaceae belonging to Clostridiales within Firmicute were the three dominant bacterial families during RSD process in most soils. Ruminococcaceae and Lachnospiraceae, rather than Clostridiaceae which was recognized as the key contributor, were tightly related to the pH decrease in RSD as an indicator of the SCFA accumulation. In addition to these mutual bacterial groups, the families Sphingobacteriales, Bacillales, Burkholderiales and Bacteroidales were also enriched in specific RSD treated soils. |
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Keywords:Soil; Bacterial community; Firmicutes; Organic materials; Anaerobic microorganisms |
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