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Correlation between SmCPS1 promoter polymorphism and tanshinones contents in Salvia miltiorrhiza
SUN Ying 1,CHEN Xin 2 *,GAN Xiaoyan 2,YAN Zhuyun 2,MU Dixiu 2,WANG Qingrong 2
1.Chengdu University of Traditonal Chinese Medicine, college of pharmacy, Chengdu 611137 China ;Chengdu University of Traditonal Chinese Medicine, college of pharmacy, Chengdu 611137 China ;Chengdu University of Traditonal Chinese Medicine, college of pharmacy, Chengdu 611137 China ;Chengdu University of Traditonal Chinese Medicine, college of pharmacy, Chengdu 611137 China ;Chengdu University of Traditonal Chinese Medicine, college of pharmacy, Chengdu 6111
2.
*Correspondence author
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Funding: National Natural Science Foundation of China (No.81573537), 自然科学基金(No.81573537)
Opened online:12 September 2017
Accepted by: none
Citation: SUN Ying,CHEN Xin,GAN Xiaoyan.Correlation between SmCPS1 promoter polymorphism and tanshinones contents in Salvia miltiorrhiza[OL]. [12 September 2017] http://en.paper.edu.cn/en_releasepaper/content/4741360
 
 
To investigate the relationship between SmCPS1 promoter polymorphism and the contents of tanshinones. We sequenced the SmCPS1 gene promoter region together with 388bp downstream from the translation start site and the content of tanshinones of 12 different germplasm of Salvia miltiorrhiza. To separate the functional polymorphic loci that may be related with the content of tanshinones from those not, the cis-elements of SmCPS1 promoter were predicted and analyzed by the Plant Transcriptional Regulatory Map database. We found 1) there was a different correlation between the polymorphism in the promoter region and the contents of the tanshinones. 2) Polymorphism on nine cis-elements, an 33bp insertion/deletion sequence and three tandem repeat variations in the sequence of SmCPS1 promoter may be related to the contents of tanshinones. These findings showed the relationships between SmCPS1 promoter polymorphism and tanshinones.
Keywords:copalyl diphosphate synthase 1; promoter; polymorphism; Salvia miltiorrhiza
 
 
 

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