| |
| 17β- Estradiol, as a major environmental estrogen, is a serious threat to human health and ecological security. The MS medium containing 0.5 mM estradiol as the sole carbon source was used to isolate a new Gram-negative bacteria from the fishing pond. This screening strain can use as the sole carbon source and energy source such as estradiol and testosterone. After 16S rRNA analysis, the bacteria belong to Acinetobacter sp with 99.71% identity. The strain was Gram-negative bacilli, Spherical, Blunt round ends, Scattered or arranged in pairs, No bud, No flagella. Size 2.126μm×0.753μm; Conditions for optimum degradation of estradiol were 27℃, 150 rpm, and substrate concentration was 1 mM/L, pH7.5 and 1% inoculum. The highest degradation rate, the degradation rate can reach 85%. The strain could grow normally in 5 μg/mL auromycin, but not in 100 μg/mL ampicillin,50 μg/mL kanamycin,10 μg/mL streptomycin. The degradation rate of 17β- estradiol was 81.4%, that of testosterone was 64.6%, that of progesterone was 44.6%, and that of 17α- ethinyl estradiol was 62.3% by high performance liquid chromatography. |
|
| Keywords:Biological detection Engineering; 16s rDNA; Estradiol degrading bacteria; High performance liquid chromatography |
|
