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A 110 nt fragment in the 5\'UTR of STAT2 that determines IRES activity and influences cell proliferation
Zhou Kantian 1,Liao Zili 2,Zhu Ruiyu 2 *
1.Laboratory of Molecular Pharmacology, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, People's Republic of China;Laboratory of Molecular Pharmacology, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, People's Republic of China;Laboratory of Molecular Pharmacology, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, People's Republic of China
2.
*Correspondence author
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Funding: none
Opened online: 7 April 2021
Accepted by: none
Citation: Zhou Kantian,Liao Zili,Zhu Ruiyu.A 110 nt fragment in the 5\'UTR of STAT2 that determines IRES activity and influences cell proliferation[OL]. [ 7 April 2021] http://en.paper.edu.cn/en_releasepaper/content/4754243
 
 
As a unique member of the STAT (Signal Transducer and Activator of Transcription) proteins family, STAT2 cannot recognize DNA target sites as homodimers like the others, yet it plays a critical role in type I interferon (IFN-I) signaling and many other signaling pathways. It was reported that STAT2 could help cells to cope with stress conditions by promoting antiviral responses in normal cells or drug resistance in carcinoma cells. As IRES (Internal Ribosome Entry Sites) were known to help promoting survival of cells under stress, together with the discovery that the 5\'UTR of STAT2 could form stable stem-loop secondary structures with a length of 203 nt, suggesting that there might be IRES activity. To testify this speculation, bicistronic reporter assay was designed as the main method to find out whether the 5\'-UTR of STAT2 harbors an IRES element or not. During the process, the IRES activity was confirmed, followed by the discovery that IRES active central domain was located at nt 33-142 in the 5\'UTR of STAT2. Afterwards, CRISPR/Cas9 techniques were utilized to construct a knockout cell line with the deletion of sequence between nt 65-129 in 5\'UTR. And it was discovered that knockout cells grew slightly faster than those wild type ones, which meant that the knocked-out fragment had an impact on normal cell proliferation. The exact mechanism behind this result remains to be further investigated.
Keywords:Biochemistry and Molecular Biology; 5\'UTR; STAT2; IRES
 
 
 

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