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QCM biosensor for detection of small nucleic acid fragments: sandwich reaction and signal amplification by silver nanoclusters
GUO Jia 1,JIANG Qifa 2,Rui-Qin Fang 1 * #
1.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054;College of Life Sciences, Sichuan Normal University, Chengdu, 610101;School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054
2.
*Correspondence author
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Funding: Research Fund for the Doctoral Program of Higher Education of China （No.No.20110185120016）, National Natural Science Foundation of China （No.No. 1201029）, Fundamental Research Funds for the Central Universities （No.No. ZYGX2014J079)）
Opened online:29 September 2021
Accepted by: none
Citation: GUO Jia,JIANG Qifa,Rui-Qin Fang.QCM biosensor for detection of small nucleic acid fragments: sandwich reaction and signal amplification by silver nanoclusters[OL]. [29 September 2021] http://en.paper.edu.cn/en_releasepaper/content/4755569
 
 
This article presents a quartz crystal microbalance (QCM) biosensor for the determination of nucleic acids by modifying electrodes with cysteine (Cys), polyethylene glycol (PEG), and silver nanoclusters (AgNCs), which represents a new approach with increased specificity and sensitivity of detection. The probe deoxyribonucleic acid immobilized on the surface of the QCM gold electrode hybridizes with a cysteine conjugate to form a specific recognition layer. The unmodified sites are then non-specifically closed by polyethylene glycol. The target gene is subsequently captured by the immobilized probe, resulting in a change in frequency. The addition of silver ions with reducing agents in an ammonia environment to form silver nanoclusters of DNA-AgNCS was used to further extend the sensitivity of the QCM sensor. The results show that it is possible to expand the frequency variation of the QCM sensor by a factor of 63.6 using such a method. A linear relationship between frequency change and DNA concentration was observed in the range of 1 nM to 10 M of target gene concentration. Thus, this biosensor provides a low-cost and sensitive tool for detecting DNA.
Keywords:QCM; Cysteine;Polyethylene glycol; Biosensors; DNA testing
 
 
 

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