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Comparative transcriptome analysis reveals the mechanism of enhanced microbial hyaluronan biosynthesis
Yao Zhiyuan 1,Gong Jinsong 2,Su Chang 2,Li Heng 2,Xu Zhenghong 2,Shi Jinsong 2 *
1.Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China ;Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China;Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China;Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China;National Engineering Research Center for Cereal Fermatation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China;Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China
2.
*Correspondence author
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Funding: the National Key Research and Development Program of China （No.No. 2021YFC2100900), the National Natural Science Foundation of China）
Opened online:13 May 2022
Accepted by: none
Citation: Yao Zhiyuan,Gong Jinsong,Su Chang.Comparative transcriptome analysis reveals the mechanism of enhanced microbial hyaluronan biosynthesis[OL]. [13 May 2022] http://en.paper.edu.cn/en_releasepaper/content/4757714
 
 
This study reveals the genetic and biochemical changes underlying the enhanced hyaluronan (HA) biosynthesis in Streptococcus zooepidemicus. The selected mutants performed a steady improvement of HA yield after multiple rounds of atmospheric and room temperature plasma (ARTP) mutagenesis combining with novel bovine serum albumin / cetyltrimethylammonium bromide coupled high-throughput screening assay. Compared to the corresponding wild type strains, the HA yield of the obtained mutant （mutant-A17） greatly increased by 42.9% with concomitant accelerated growth. Transcriptome sequencing exhibits that distinct mutants have similar genetic changes. Regulation in direction of metabolic flow into the HA biosynthesis, by enhancing genes responsible for the biosynthesis of HA including hasB, glmU and glmM, weaking downstream gene (nagA and nagB) of UDP-GlcNAc and significantly down-regulating transcription of wall-synthesizing genes, which could cause accumulation of precursors. These associated regulatory genes may provide control point for the efficient synthesis of HA and engineering of the HA-producing cell factory.
Keywords:Hyaluronic acid, Atmospheric and room temperature plasma, Streptococcus zooepidemicus, Comparative transcriptome analysis, Metabolic pathway
 
 
 

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