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Screening of strains from reconstituted tobacco sheet making high-yield enzymes and aroma
Yang Chunxue,Deng Zhang,Liao xiang ru,Guan Zhengbing *
Biotechnology School, Jiangnan University, Wuxi 214122;Taicang Haiyan Tobacco Flakes Co., Ltd., Shanghai Tobacco Group, Suzhou 215000;Biotechnology School, Jiangnan University, Wuxi 214122;Biotechnology School, Jiangnan University, Wuxi 214122
*Correspondence author
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Funding: National Natural Science Foundation （No.Grant No. 31472003）
Opened online: 7 March 2023
Accepted by: none
Citation: Yang Chunxue,Deng Zhang,Liao xiang ru.Screening of strains from reconstituted tobacco sheet making high-yield enzymes and aroma[OL]. [ 7 March 2023] http://en.paper.edu.cn/en_releasepaper/content/4759360
 
 
Reconstituted tobacco sheet (RTS) is critical to reduce the harm of cigarettes and considered as a comprehensive utilization approach for tobacco wastes in the tobacco industries. The aim of this study was to screen out bacteria from RTS materials capable of producing a high total amount of enzymes and aroma. A total of 21 strains with unique morphologies were obtained. Based on the results of enzymes and aroma producing capacities of the strains, one strain was screened. This strain, which was named B-NS-7, was capable of producing a total amount of 696.3 U/mL protease activity, 238.4 U/mL amylase activity, 64.3 U/mL pectinase activity and 100.0 μg/mL aroma. Combined with the results of the 16S rDNA sequencing, physiological and biochemical characteristics B-NS-7 was identified as Bacillus subtilis. Through a series of single factor experiences, the results showed that under the conditions of inoculation concentration of 1%, fermentation temperature of 37 C and time of 24 h, the highest aroma content of concentrated solutions were 116.3 μg/mL. Neophytadiene was responsible for the majority of the fragrance enhancement, and B-NS-7 was identified as an aldehydic and alkene-flavoured strain. The sensory quality of tobacco concentration solutions was superior under optimal conditions. This strain has been deposited at the China General Microbiological Culture Collection Center (CGMCC No. 23716). By way of this study, B. subtilis B-NS-7 was intended to be used in the manufacturing of RTS through microbial fermentation to improve the quality and providing suggestions for the microbial development of the RTS industry.
Keywords:Reconstituted tobacco sheet; Function microbial; Fragrance; Fermentation; Screening
 
 
 

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