Factors affecting plant regeneration from cotyledon explants of cultivated pepper (Capsicum annuum L.) species

Zhang Yuyang; Tang Yinzhu; Ye Zhibiao; Lu Yongen; Ouyang Bo; Zhang Chanjuan; Li Hanxia

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Factors affecting plant regeneration from cotyledon explants of cultivated pepper (Capsicum annuum L.) species

Zhang Yuyang * #,Tang Yinzhu,Ye Zhibiao,Lu Yongen,Ouyang Bo,Zhang Chanjuan,Li Hanxia

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University

*Correspondence author

#Submitted by

Subject:

Funding: none

Opened online:21 February 2006

Accepted by: none

Citation: Zhang Yuyang,Tang Yinzhu,Ye Zhibiao.Factors affecting plant regeneration from cotyledon explants of cultivated pepper (Capsicum annuum L.) species[OL]. [21 February 2006] http://en.paper.edu.cn/en_releasepaper/content/5284

A detailed study was carried out to investigate key factors affecting bud induction and shoot elongation of pepper regeneration, and develop a reliable and highly efficient in vitro regeneration system. Ability for in vitro shoot regeneration and elongation is genotype dependent. “Chufeng” displayed the highest regeneration response: 100% frequency of adventitious bud induction with all hormone combinations tested. The fully expanded cotyledons of 8–12-day-old seedlings gave the highest regeneration efficiency. Adventitious buds subjected to 21-day-induction produced higher elongation efficiency than that of 14-day-induction. The hormone combination of Zeatin + gibberellic acid generated the most efficient bud elongation. The media for in vitro regeneration from cotyledon explants of pepper were optimized: the buds induction medium: Murashige and Skoog medium+ 6-benzyl-aminopurine 6.0 mg l–1 + indole-3-acetic acid 1.0 mg l–1; the shoots elongation medium: Murashige and Skoog salts + B5 vitamins + Zeatin 2.0 mg l–1 + gibberellic acid 1.5 mg l–1; the rooting medium: half-strength MS + 0.5 mg l–1 indole-3-butyric acid. Influence of other factors such as Ag+ and seedling nutrient juice on regeneration was also studied. In general, high frequency of regeneration was obtained from all varieties tested. Plantlets were transplanted to soil and acclimatized in the greenhouse showing normal development and grown to maturity bearing normal fruits with seeds. An overall optimized scheme for pepper regeneration in vitro from cotyledons was presented, through which 100% bud induction and 50%–100% elongation efficiency was obtained.

Keywords:Capsicum annuum L., plant regeneration, organogenesis, factor optimization, cotyledon

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