Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse-phrase High Performance Liquid Chromatography Under Salt and Alkali Stress

Hong Yan

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Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse-phrase High Performance Liquid Chromatography Under Salt and Alkali Stress

Hong Yan #

School of Life Sciences,Northeast Normal University

*Correspondence author

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Funding: none

Opened online:20 September 2006

Accepted by: none

Citation: Hong Yan.Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse-phrase High Performance Liquid Chromatography Under Salt and Alkali Stress[OL]. [20 September 2006] http://en.paper.edu.cn/en_releasepaper/content/8417

Several organic acids accumulated in Kochia Scoparia shoots and roots were studied by means of reverse-phrase high performance liquid chromatography with A C18 column. Five types of binary organic acids were separated. The organic acid concentrations were determined in K. Scoparia seedlings stressed by saline (NaCl) and alkaline (NaHCO3) at the same Na+ concentration. Concentrations of organic acids were stimulated by alkaline because the cells will adjust their pH values through the accumulation of organic acids, when the environment is basic. The concentrations of oxalic acid and succinic acid are higher than those of other organic acids, including tartaric acid and malic acid, and the concentration of citric acid is the lowest. The concentrations of organic acids in roots are higher than those in shoots under salt (NaCl) stress, but he results are opposite while the roots are under alkali (NaHCO3) stress. This indicates that there are different adaptive strategies for K. Scoparia seedlings in organic acid metabolism under salt and alkali stress

Keywords:Kochia Scoparia (L.) Schrad; Salt stress; Alkaline stress; Organic acid; HPLC

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