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| Enantiomers of 5-Methyl-5-phenylhydantoin (MPH) were separated on norvancomycin chiral stationary phase (NVC-CSP), which was prepared by covalently linking norvancomycin to acid-treated silica gel. Three mobile phase modes have been investigated: reversed phase, polar organic mode, and normal phase. MPH may be enantioseparated under the reversed phase and polar organic mode. There is no enantioseparation under the normal phase. The effects of composition, column temperature, pH, and flow rate of mobile phase on the enantioseparation were investigated. The enantioseparation by methanol/2% TEAA (pH 5.5) 10/90 at the flow rate 0.6 ml/min and 20篊 is excellent. The hydrophobic interactions and hydrogen bonding are the dominant interactions on enantioseparations between the analyte and macrocycle in this chromatographic system. |
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| Keywords:5-Methyl-5-phenylhydantoin; Norvancomycin; Enantioseparation; Chiral stationary phase |
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