Identification, characterization and gene cloning of a phytase with potential industrial interest

Ming-Lv Sun; Bo Zhou; Jian Sun; Dong-Min Zhao; Xiao-Yun Wang

Chinese︱Feedback︱Save this page

• Elaborating Academic Views 　　　　 • Exchanging Innovative Ideas
• Protecting Intellectual Properties 　　• Fast Sharing Science Papers

Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China

Home > Papers

Identification, characterization and gene cloning of a phytase with potential industrial interest

Ming-Lv Sun,Bo Zhou,Jian Sun,Dong-Min Zhao,Xiao-Yun Wang *

College of Life Science, Shandong Agricultural University

*Correspondence author

#Submitted by

Subject:

Funding: China Natural Science,RESEARCH Fund for Doctoral Program of Higher（No.30471261,20050434008）

Opened online:12 April 2007

Accepted by: none

Citation: Ming-Lv Sun,Bo Zhou,Jian Sun.Identification, characterization and gene cloning of a phytase with potential industrial interest[OL]. [12 April 2007] http://en.paper.edu.cn/en_releasepaper/content/12158

A high phytase-producing strain of Aspergillus niger N-3 was identified by screening 94 microbial strains. The phytase displayed physicochemical characteristics of potential industrial interest with independent intellectual property. The enzyme activity in fermented broth achieved a maximum of 495 U/mL. The enzyme was purified by a combination of ammonium sulfate fractionation and gel filtration chromatography. The molecular weight of the enzyme as determined by SDS-PAGE was 60-80 kDa, with maximum activity at about 55 C (after incubation at 10 min). Dual optima pH (pH 2.0 and pH 5.5) was gained. Activity at pH 2.0, which is more suitable to the circumstance of monogastric animals’ stomach was about 30% higher than that at pH 5.5. The phytase retained about 45% of its enzymatic activity under heat treatment at 90 C for 5 min. It showed a greater affinity for sodium phytate (Km = 132.3 μM) than for pNPP (Km = 2.23 mM). The phyA gene was isolated and sequenced. The coding region without the introns and putative signal sequence was comprised of 1347 nucleotides. It encoded a polypeptide of 448 amino acids， exhibiting high amino acid sequence homologies (94.87%) with the typical phytase Aspergillus niger NRRL 3135.

Keywords:Phytase; Strain Screening; Enzymatic property; Gene Cloning

For this paper

● PDF (0B)
● Revision 0 　　
● Print this paper
● Recommend this paper to a friend
● Add to my favorite list

Saved Papers

Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded	1022
Bookmarked	1
Recommend	5
Comments	Array

Submit your papers

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.

	Check out RSS, or use RSS reader to subscribe this item

Saved Papers