Development of Trichoderma reesei cbh1 promoter to improve heterologous gene expression

Ti Liu; Tianhong Wang; Xian Li; Xuan Liu

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Development of Trichoderma reesei cbh1 promoter to improve heterologous gene expression

Ti Liu 1,Tianhong Wang 1 * #,Xian Li 2,Xuan Liu 2

1.State Key Laboratory of Microbial Technology, Shandong University

2.State Key Laboratory of Microbial Technology, Shandong University

*Correspondence author

#Submitted by

Subject:

Funding: 国家自然科学基金,教育部博士点基金,973课题,973课题（No.30470052, 20040422042,2003CB716006,2004CB719702）

Opened online:30 August 2007

Accepted by: none

Citation: Ti Liu ,Tianhong Wang,Xian Li.Development of Trichoderma reesei cbh1 promoter to improve heterologous gene expression [OL]. [30 August 2007] http://en.paper.edu.cn/en_releasepaper/content/14772

To improve heterologous gene expression in Trichoderma reesei, a set of optimal artificial cbh1 promoters were obtained. The -677～-724 region with three potential glucose repressor binding sites was deleted. And then the -620～-820 region of the modified cbh1 promoter including the CCAAT box and Ace2 binding site was repeatedly inserted into the modified cbh1 promoter, obtaining promoters with copy number 2, 4 and 6, respectively. The experimental results showed that the glucose repression effects were abolished and the glucuronidase (GUS) reporter gene expression guided by these multi-copy promoters was markedly enhanced as copy number increased simultaneously. The date showed the great promise of utilizing promoter artificial modification strategy to increase heterologous gene expression in filamentous fungi and provided a set of optional high-expression vectors for gene function investigation and strain modification.

Keywords:Trichoderma reesei；cbh1 promoter；carbon catabolite derepression；targeted deletion；multiple-copy strategy

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