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| Purposes: To investigate the distribution of human papillomavirus (HPV) genotypes in uterine cervical lesions in Yanbian of Northern China. Materials and Methods: Paraffin embedded blocks of 62 cases of squamous cell carcinomas (SCC), 28 cases of adenocarcinomas, and 143 cases of CIN (CIN-1: 45; CIN-2: 46; CIN-3: 52) were selected from Dept. of Pathology, Yanbian University Hospital and Yanbian Women’s Hospital in the period of 2000-2005. DNA was extracted by using High Pure PCR Template Preparation Kit from all above blocks. And HPV genotypes were detected by using oligonucleotide microarray (HPV DNA-chip). Results: All the 20 cases of normal cervical epithelia are negative for HPV by both TaKaRa PCR and DNA-chip methods. The positive rate of high-risk HPV is 28.9% in CIN-1, 41.3% in CIN-2, 44.2% in CIN-3, 85.5% in SCC, and 82.1% in adenocarcinoma by TaKaRa PCR method. Similarly, it is 26.7% in CIN-1, 41.3% in CIN-2, 40.4% in CIN-3, 82.3% in SCC, and 78.6% in adenocarcinoma by HPV DNA-chip. HPV 16 is the major type in all CIN and SCC cervical lesions. However, in cervical adenocarcinoma, HPV 18 is the most common type (59.1% in HPV positive cases), and HPV 16 is the second type, but still show a high percentage (31.8% in HPV positive cases). There is small number of multi-types HPV detected in CIN-3, SCC, and adenocarcinoma, but none in CIN-1/2. Meanwhile, there is no significant difference on the HPV screening between TaKaRa PCR and HPV DNA-chip methods (p<0.05). Conclusions: HPV 16 is the type most frequently involved in the development of SCC of the cervix, whereas both HPV 18 and 16 play a prominent role in the development of adenocarcinoma of the cervix in Yanbian of Northern China. Both TaKaRa PCR and HPV DNA-chip methods are sensitive for HPV detection, and can be used for the screening of HPV infection status and genotyping in uterine cervical lesions, and maybe helpful for the prediction of the development and progress of CIN-2/3.
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| Keywords:Papillomavirus, Human; Uterine Cervix; Genotyping |
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