Influence of conformational changes on the kinetics behavior in hydrolysis of cellulose I and cellulose II&frac34; Acid Vs Enzymatic

Wu Bin; Wang LuShan; Zhao Yue; GAO Peiji

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Influence of conformational changes on the kinetics behavior in hydrolysis of cellulose I and cellulose II¾ Acid Vs Enzymatic

Wu Bin 1 #,Wang LuShan 1,Zhao Yue 2,GAO Peiji 2

1.State key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 PR China

2.State key Laboratory of Microbial Technology- Shandong University

*Correspondence author

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Funding: 教育部博士点基金（No.2003.0422024）

Opened online: 7 March 2005

Accepted by: none

Citation: Wu Bin,Wang LuShan ,Zhao Yue.Influence of conformational changes on the kinetics behavior in hydrolysis of cellulose I and cellulose II¾ Acid Vs Enzymatic[OL]. [ 7 March 2005] http://en.paper.edu.cn/en_releasepaper/content/1650

Cellulose II prepared by mercerizing cotton fibers (cellulose I) is thought to be a useful polymer. Studies show that the hydrolysis of cellulose II by dilute acid is more remarkable than that of cotton fibers, whereas it is as difficult to be hydrolyzed by cellulase as cotton fibers. According to the structural changes such as swell of fibrils and decrease of Cr I % (Crystallinity index) confirmed by Atom force Microscopy (AFM, Atom force Microscopy), X-ray diffraction analysis and FT-IR (Transform-infrared spectrum) spectral analysis, cellulose II should show more susceptibility for both acid and enzymatic hydrolysis. However the crystal structure in cellulose II is more stable and the potential energy is to be higher than those of cotton fibers, which may be the key factors contributing to the decrease of hydrolysis rate by cellulase.

Keywords:cellulose II, cellulase, crystal

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