Effect of Calnexin Deletion on the Expession Level of Binding Protein (BiP) Under Heat Stress Conditions in Saccharomyces cerevisiae

Zhang Huili; Hu Bingjie; Ji Yanyan; Akio Kato; Song Youtao

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Effect of Calnexin Deletion on the Expession Level of Binding Protein (BiP) Under Heat Stress Conditions in Saccharomyces cerevisiae

Zhang Huili 1,Hu Bingjie 1,Ji Yanyan 1,Akio Kato 2,Song Youtao 1 * #

1.College of Life Science, Liaoning University

2.Department of Biological Chemistry, Yamaguchi University

*Correspondence author

#Submitted by

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Funding: 辽宁省教育厅,辽宁省教育厅,沈阳市科技局（No.20060358,05L156,1063305-1-00）

Opened online:14 December 2007

Accepted by: none

Citation: Zhang Huili ,Hu Bingjie ,Ji Yanyan .Effect of Calnexin Deletion on the Expession Level of Binding Protein (BiP) Under Heat Stress Conditions in Saccharomyces cerevisiae[OL]. [14 December 2007] http://en.paper.edu.cn/en_releasepaper/content/16921

In order to investigate the effect of calnexin deletion on the induction of main ER molecular chaperone BiP, we cultured the wild-type and calnexin-disrupted Saccharomyces cerevisiae strains under the normal and stress conditions. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced mRNA level of BiP in the ER was evidently higher in calnexin-disrupted S. cerevisiae relative to the wild type at 37 C, but was almost the same in the two strains under the normal conditions. The western blotting analysis for BiP protein expression in the ER yielded results that showed a parallel in their mRNA levels in both strains. It is suggested that under heat stress conditions the induction of BiP in the ER could recover parts of the function of calnexin in calnexin-disrupted yeast, and result in the same growth rate as in wild-type yeast.

Keywords:Calnexin, Molecular chaperone, BiP, Heat stress

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