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Biocontrol of Alternaria alternata on Cherry Tomato Fruit by Use of marine yeast Rhodosporidium paludigenum Fell & Tallman
Wang Yifei ,Feng Wu,Shen Danhong,Yu Ting ,Zheng Xiaodong * #
Department of Food Science and Nutrition, Zhejiang University
*Correspondence author
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Funding: 教育部博士点基金(No.20040335025)
Opened online:24 December 2007
Accepted by: none
Citation: Wang Yifei ,Feng Wu,Shen Danhong.Biocontrol of Alternaria alternata on Cherry Tomato Fruit by Use of marine yeast Rhodosporidium paludigenum Fell & Tallman[OL]. [24 December 2007] http://en.paper.edu.cn/en_releasepaper/content/17308
 
 
The vasidiomycetous yeast Rhodosporidium paludigenum Fell & Tallman, which was isolated from the south of East China Sea was evaluated for its activity in reducing postharvest decay of cherry tomatoes caused by Alternaria alternata in vitro and in vivo test. The result showed that washed cell suspensions of R. paludigenum provided better control of A. alternata than other treatments. Autoclaved cell culture failed to provide protection against the pathogen. The concentration of antagonist had significant effect on biocontrol effectiveness. When the washed yeast cell suspension reached the concentration of 1×109 CFU/ml and challenged with pathogen spore suspension at 5×104 spores/ml, the rate of decay was only 37% after 5-day incubation at 25℃. Efficacy of R. paludigenum was obvious when it was applied simultaneously or prior to inoculation with A. alternata. There was little efficacy when R. paludigenum was applied after A. alternata inoculation. The longer incubated time of R. paludigenum was, the lower disease incidence would be. When the incubation time was reached 54 h, the decay was only 33%. On the wounds of cherry tomato, the population of R. paludigenum increased 50-fold during the first 12 h at 25℃. The maximum marine yeast population was nearly 100-fold over the original ones after 24 h.
Keywords:Cherry tomato; Biological control; Alternaria alternata; Rhodosporidium paludigenum Fell & Tallman; Marine yeast
 
 
 

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