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| We tried to find an anti-angiogenesis component from Tupistra Cinensis Bak., a natural medicinal herb in China, using the ECV304 cell membrane chromatography (CMC) combined with chicken chorioallantoic membrane (CAM) assay. Samples were obtained using ethanol extraction, a resin column and a normal-phase column separation from Tupistra Cinensis Bak. Samples were screened by an ECV304 CMC model with the anti-flt-1 antibody as the control molecule. An active component that acted on the ECV304 cell membrane receptor (e.g., VEGFR) was found. The structure of this component was determined by UV, IR, MS and NMR and named as (5?-oleandrigenin-3-(?L-rhamnopyranoside) or ODGR. The pharmacological effect of ODGR was tested by a chicken CAM neovascularization model in vivo. ODGR significantly inhibited CAM angiogenesis within 0.94?5 礸/egg. CAM histomorphological results indicated that ODGR could inhibit sprouting of blood vessels and proliferation of vascular endothelial cells. These findings suggest that ODGR is a promising inhibitor of angiogenesis. The method of ECV304 CMC combined with chicken CAM assay can be used for discovering compounds with anti-angiogenesis actions from natural medicinal herbs. |
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| Keywords:Tupistra Cinensis Bak.;anti-angiogenesis;ECV304 cell membrane chromatography (ECV304 CMC);Chicken chorioallantoic membrane (Chicken CAM);(5?-oleandrigenin-3-(?L-rhamnopyranoside) |
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