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| To investigate the interaction effects of promoter region A163G,T245G and T950C polymorphisms of osteoprotegerin(OPG) gene and calcium intake on bone mass in premenarche girls. Methods: The OPG genotypes were determined by PCR-RFLP in 214 premenarche girls of Han race in Guangzhou area, and bone mineral density(BMD) at lumbar spine(L1-4), femoral neck, Ward’s triangle area(WSBMD) and total BMD, were measured by dual-energy X-rayabsorptiometry(DEXA). Current dietary calcium intakes were assessed by 3-day food record questionnaire and 3-day weighed food record method. Results: Hardy-Weinberg equilibrium was evident for OPG polymorphisms. After adjusting the confounding factors of year, height and weight, ANCOVA showed significant effect of interaction of calcium intake and T950C SNPs on WSBMD(P=0.046). WSBMD of subjects in calcium intake<500mg/d group was lower 13.0% 、23.7% than those in 500~800mg/d group and >800 mg/d group respectively among girls with TT genotype of T950C locus. However, such significance was not detected among girls with TC or CC genotype of T950C locus. Significant interaction was not observed between A163G、T245G SNPs and calcium intake on BMD. Conclusion: calcium intake and OPG T950C SNP has significant interaction on WSBMD in premenarche girls. More calcium intake would be benefit to gain higher WSBMD for girls with TT genotype of T950C locus than those with TC or CC genotype |
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| Keywords:OPG;PCR-RFLP;Calcium intake;Interaction |
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