Complete nucleotide sequences of two double-stranded RNAs from Raphanus sativus-root demonstrate the incidence of a novel cryptic virus

Qinghua Tian; Liqing Li; Jishuang Chen

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Complete nucleotide sequences of two double-stranded RNAs from Raphanus sativus-root demonstrate the incidence of a novel cryptic virus

Qinghua Tian #,Liqing Li,Jishuang Chen *

Institute of Bioengineering, Zhejiang Sci-Tech University

*Correspondence author

#Submitted by

Subject:

Funding: 国家自然科学基金（No.30671361）

Opened online:26 February 2009

Accepted by: none

Citation: Qinghua Tian,Liqing Li,Jishuang Chen.Complete nucleotide sequences of two double-stranded RNAs from Raphanus sativus-root demonstrate the incidence of a novel cryptic virus [OL]. [26 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29713

Two double-stranded RNAs were extracted from leaf tissues of Raphanus sativus-root cv. Yidianhong grown in Eastern China. Their full sequences, namely RasR7 and RasR8, were determined by a single primer amplification technique. Comparisons of 5′ untranslated regions (UTR) and 3′ UTR between RasR7 and RasR8 showed that they were highly conserved at 5′ UTR and an identical region 5′-AGAAUUU-3′ was discovered as the evidence of the same plant virus. 5′-UAAGAC-3′ was found as their identical region in 3′ UTR. BLASTP search in Genbank showed that RasR7 encoded putative protein was much similar with RNA dependent RNA polymerases (RdRps) of plant-infecting dsRNA viruses belonging to the family Partitiviridae. Phylogenetic analysis showed that RasR7 encoded putative protein clustered with the RdRps of members of the family Partitiviridae. RT-PCR detection indicated that both RasR7 and RasR8 existed in the virus-like particles. Therefore, it was suggested that RasR7 and RasR8 institute the genome of a newly discovered cryptic virus, namely Raphanus sativus cryptic virus 3 (RasV3).

Keywords:double-stranded RNA;Raphanus sativus;RdRp;sequence analysis;dsRNA virus

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