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Interaction of BSA and long-chain imidazolium ionic liquid measured by fluorescence spectra and surface tension
Fei Geng 1,Liqiang Zheng 2,Li Yu 2 * #,Ganzuo Li 2,Chenho Tung 3
1.Key Laboratory of Colloid and Interface Chemistry,Shandong University, Ministry of Education
2.Key Laboratory of Colloid and Interface Chemistry,Shandong University, Ministry of Education
3.Technical Institute of Physics and Chemistry,The Chinese Academy of Sciences
*Correspondence author
#Submitted by
Subject:
Funding: 国家自然科学基金,山东省自然科学基金,教育部博士点基金（新教师基金）（No.20773081,Z2007B03,070422047）
Opened online:23 July 2009
Accepted by: none
Citation: Fei Geng,Liqiang Zheng,Li Yu.Interaction of BSA and long-chain imidazolium ionic liquid measured by fluorescence spectra and surface tension[OL]. [23 July 2009] http://en.paper.edu.cn/en_releasepaper/content/34047
 
 
The binding of long-chain imidazolium ionic liquid (IL), 1-tetradecyl-3-methylimidazolium bromide (C14mimBr) to bovine serum albumin (BSA) were investigated by fluorescence spectra and surface tension. Fluorescence spectra show that tryptophan (Trp) residues, one of the intrinsic fluorophores in BSA, are buried to a hydrophobic microenvironment with the addition of C14mimBr. The addition of C14mimBr induces denaturation of BSA. Moreover, the fluorescence quenching mechanism was determined to be static quenching. The equilibrium constant (K) and the number of binding sites (n) were calculated based on the results of fluorescence measurement. According to the surface tension plots, the critical aggregation concentration (CAC) and critical micelle concentration (CMC) under different BSA concentrations at various temperatures were investigated. The surface tension indicates that C14mimBr binds to BSA through electrostatic attraction at low concentration of C14mimBr (below CMC) and through hydrophobic interaction at high concentration of C14mimBr (above CMC). Additionally, thermodynamic parameters of micelle formation were determined. This study provides an understanding of the binding of C14mimBr to BSA.
Keywords:Bovine serum albumin;long-chain imidazolium ionic liquid;fluorescence spectra;surface tension;interaction
 
 
 

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