Effect of silver nitrate on callus regeneration and activity of some enzymes during tissue culture of wheat immature inflorescence

Xiangru Liao; Lei Tang; Feng Zhang

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Effect of silver nitrate on callus regeneration and activity of some enzymes during tissue culture of wheat immature inflorescence

Xiangru Liao * #,Lei Tang,Feng Zhang *

Southern Yangtze University

*Correspondence author

#Submitted by

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Funding: 河北省博士基金（No.无）

Opened online: 1 November 2005

Accepted by: none

Citation: Xiangru Liao,Lei Tang,Feng Zhang.Effect of silver nitrate on callus regeneration and activity of some enzymes during tissue culture of wheat immature inflorescence[OL]. [ 1 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3441

Effects of silver nitrate on callus regeneration and activity of indole-3-acetic acid oxidase and peroxidase, glutathione S-transferase and reductase, aldehyde oxidase, catalase and peroxidase in cells of immature inflorescence of Triticum aestivum L. variety JI135 were investigated. Immature inflorescences with length between 5-10mm were treated with AgNO3 in MS medium in a period of 10 days. The results indicated that callus regeneration was improved by treatment of wheat immature inflorescence with 16 mg/L AgNO3 for 7 days. The activity of indole-3-acetic acid oxidase and peroxidase was reduced obviously by 16 mg/L AgNO3 four days and six days after inoculation respectively, activity of glutathione transferase， glutathione reductase，catalase，and peroxidase were obviously promoted by AgNO3 4 days after the treatment, But changes in activity of the aldehyde oxidase was not significantly influenced by AgNO3. The mechanism of AgNO3 in improving cell regeneration was discussed.

Keywords:AgNO3; catalase; glutathione S-transferase and reductase; indole-3-acetic acid oxidase and peroxidase; peroxidase; Callus; Regeneration; Triticum aestivum L.

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