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Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China
A novel monitoring technique series piezoelectric quartz crystal (SPQC) sensing technique is used to monitor conductivity in unfolding and refolding processes of lysozyme in real time. The lysozyme is unfolded by sodium dodecylbenzene sulfonate (SDBS) and then refolded by cetyltrimethylammonium bromide (CTAB). During the unfolding and refolding process, the conductivity of the solution changed accordingly. The change in conductivity caused a sensitive response of the SPQC sensor. The obtained experimental results indicate that the SPQC sensing technique would be a useful and convenient tool for monitoring the interaction between ionic surfactants and protein.