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Reversible Conduction Block in Isolated Toad Sciatic Nerve by Emulsified Isoflurane
Li Zhuo 1,Yang Jing 2 *,Liu Jin 3,Gong Chunyu 4,Gan Jing 5,Zhang Xian 5,Luo Wenjun 5,Li Guohua 5
1.Department of Pharmacology,West China Medical School,Sichuan University
2.Laboratory of Anaesthesia and CCM,West China Hospital,Sichuan University
3.Laboratory of Anaesthesia and CCM,West China Hospital, SCU
4.Department of Urology, The Second Hospital of Chengdu
5.Laboratory of Anaesthesia and CCM,West China Hospital
*Correspondence author
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Funding: 教育部博士点基金,国家自然科学基金（No.20060610062,30700782）
Opened online:25 December 2009
Accepted by: none
Citation: Li Zhuo,Yang Jing ,Liu Jin .Reversible Conduction Block in Isolated Toad Sciatic Nerve by Emulsified Isoflurane[OL]. [25 December 2009] http://en.paper.edu.cn/en_releasepaper/content/38098
 
 
Background: Studies have shown that the local use of volatile anesthetics can produce some local anesthetic effects. The present study was designed to evaluate characteristics of nerve conduction block of emulsified isoflurane (EI), and compare its nerve blockade with 1% lidocaine, by measuring compound nerve action potential (CNAP) parameters in isolated toad sciatic nerve. Methods: One hundred isolated toad sciatic nerves were selected and randomly assigned to 10 groups of 10 each, receiving administration of 2-8% EI (v/v) (EI8 group, etc), 1% lidocaine (Lido group), 30% Intralipid?(Lipid group) and Ringer?solution (RS group) for 10min, respectively. Then all nerves were washed and soaked by RS for 10min and 30min. The nerve conduction block effect was represented by CNAP parameters which were recorded by extracellular recording technique per min. Results: The results showed that the negative amplitudes of CNAP were decreased by EI as well as Lidocaine (P < 0.05), and the conduction velocities of CNAP were also decreased in some time points (D7-W3) (P < 0.05). After RS washing, the two parameters recovered gradually. The changes in the two parameters induced by EI had slower onset rates and faster recoveries than those produced by lidocaine (7 min vs. 1 min, 9 min vs. 30 min). The nerve blockade induced by EI was dose-dependent (P < 0.05), and the half maximal inhibition concentration of EI was 5.46 %. Conclusions: EI produced completely reversible and dose-dependent nerve conduction inhibition, which had slower onset and faster recovery compared to those produced by lidocaine.
Keywords:Emulsified Isoflurane;Compound nerve action potential;Local anesthetics;Isolated toad sciatic nerve
 
 
 

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