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| Potato tuberization is a unique biologic process in Solanaceous plants. However, a few are known yet on which genes are involving in metabolism of the process. To dig genes related to tuberization in full transcriptome wide, with leafs and stems/stolons of the early- and late-mature genotypes, 4 vertical and 7 horizontal cDNA subtraction hybridizations were done and led to 11 cDNA libraries constructed. Libraries screening, ESTs sequencing, Reverse Northern Blotting and database search revealed that 217 unigenes from nucleus, choroplasm and mitochondrial genome expressed in leafs and stems/stolons related to tuberization. Data analysis indicated that 1) glucose metabolism companying by a strong redox activation in leafs, a week redox and catalysis level in stems leads to a late formation of stolon. 2) A strong redox activation resulting from lipid and protein metabolisms in stems and a low level of catalysis in leaf is necessary for a early stolon formation.3) it is dehydrin, an unique recognized metabolite, occurring in stems after, rather than before tuberzation. As a conclusion, changes of redox reactions and metabolism lead to either no or early/late tuberization. All above is identical with experiment conclusions reported and deepen our understanding of stolon and tuber formation. |
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| Keywords:cDNA;unigenes;tuberization;Potato;Solanacae |
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